Transduction of primary rat hepatocytes with bicistronic retroviral vector
نویسندگان
چکیده
منابع مشابه
Retroviral vector infection and transplantation in rats of primary fetal rat hepatocytes.
Retroviral vectors were used to transduce recombinant DNA encoding firefly luciferase, Escherichia coli beta-galactosidase or human factor IX into fetal rat hepatocytes in primary culture. Hepatocytes were transduced optimally during a restricted time interval, 2-4 days post-plating. Although efficient and stable expression of reporter gene products was observed in vitro, it was affected differ...
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BACKGROUND Internal Ribosome Entry Site (IRES)-based bicistronic vectors are important tools in today's cell biology. Among applications, the expression of two proteins under the control of a unique promoter permits the monitoring of expression of a protein whose biological function is being investigated through the observation of an easily detectable tracer, such as Green Fluorescent Protein (...
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Hepatic gene therapy might correct the clinical manifestations of several genetic disorders in patients. Although retroviral vectors with a strong liver-specific promoter can result in stable and therapeutic levels of expression of genes from the liver, application of these techniques in humans is limited by the need to perform one or more invasive procedures to achieve ex vivo or in vivo trans...
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in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...
15 صفحه اولFunctional testing of a bicistronic retroviral vector for intracellular peptide production.
Cytotoxic T cells generally recognize short 8-mer to 11-mer peptides in association with major histocompatibility complex (MHC) class I molecules on the surface of the antigen presenting cells (APCs). Usually, these peptides are produced in the cytosol of the APCs by proteolytic degradation of cytosolic proteins. The peptides are subsequently transported first to the endoplasmatic reticulum (ER...
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ژورنال
عنوان ژورنال: World Journal of Gastroenterology
سال: 2000
ISSN: 1007-9327
DOI: 10.3748/wjg.v6.i5.725